which of the following phases of mitosis is characterized by the separation of sister chromatids and their movement to opposite poles
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1. Which of the following phases of mitosis is characterized by the separation of sister chromatids and their movement to opposite poles?

Correct answer: B

Rationale: During anaphase of mitosis, the sister chromatids, which are duplicated copies of a chromosome, separate and move towards opposite poles of the cell. This movement is facilitated by the shortening of microtubules attached to the chromatids. As a result, each pole of the cell receives a complete set of chromosomes, ensuring that the daughter cells produced after cell division will have the correct number of chromosomes. Metaphase is characterized by the alignment of chromosomes at the cell's equator, not their separation. Telophase is the phase following anaphase, where the separated chromatids reach the opposite poles and nuclear envelopes start to form around them. Cytokinesis is the final stage of cell division, where the cytoplasm is divided to form two separate daughter cells.

2. Which of the following structures is unique to eukaryotic cells?

Correct answer: B

Rationale: Nuclei are structures that are unique to eukaryotic cells. Prokaryotic cells lack a defined nucleus, and their genetic material floats freely in the cytoplasm. Eukaryotic cells have nuclei that house the genetic material in the form of chromosomes, separated from the cytoplasm by a nuclear membrane. This distinct organelle is a key feature that sets eukaryotic cells apart from prokaryotic cells. Cell walls (Choice A) are found in plant cells, fungi, and some prokaryotes but are not unique to eukaryotic cells. Cell membranes (Choice C) are present in both prokaryotic and eukaryotic cells, serving as a barrier that encloses the cell contents. Vacuoles (Choice D) are membrane-bound organelles found in both plant and animal cells, making them not unique to eukaryotic cells.

3. How does the Pauli exclusion principle relate to the structure of the atom?

Correct answer: A

Rationale: The Pauli exclusion principle states that no two electrons in an atom can have the same set of quantum numbers. This principle directly influences the structure of the atom by defining the maximum number of electrons allowed in each energy level. As a result, it helps determine the electron configuration and the arrangement of electrons in different orbitals within an atom. Choices B, C, and D are incorrect as they do not directly relate to the Pauli exclusion principle's specific impact on the electron distribution within an atom.

4. In order to verify if a particular drug is responsible for the recovery of individuals with a disorder, what procedure would be most effective in testing its effectiveness?

Correct answer: A

Rationale: The most effective procedure in testing whether the drug is responsible for the recovery is to ensure that neither the subjects nor the investigators know which subjects are given the drug and which are given the placebo. This method is known as a double-blind study, which helps eliminate biases and ensures that the results are reliable and not influenced by expectations or beliefs. By keeping both the subjects and investigators unaware of who is receiving the drug or placebo, the study can accurately determine the true impact of the drug on recovery. Choice B is incorrect as it only prevents the subjects from knowing which treatment they are receiving, allowing for potential bias from the investigators. Choice C is also incorrect as it allows for potential bias from the subjects. Choice D is incorrect as full knowledge of treatment allocation can introduce bias and affect the study's validity.

5. Which of the following enzymes unwinds the double-stranded DNA during replication?

Correct answer: A

Rationale: Helicase is the enzyme responsible for unwinding the double-stranded DNA during replication by breaking the hydrogen bonds between the base pairs. This process creates the replication fork where DNA polymerase can then synthesize new DNA strands. Ligase functions to join Okazaki fragments on the lagging strand, not unwind DNA. Nuclease is involved in DNA repair by removing damaged DNA segments. Polymerase is responsible for synthesizing new DNA strands based on the existing template strands, not for unwinding the DNA.

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