what is the crispr cas9 system used for in gene editing

ATI TEAS 7

TEAS Test 7 science quizlet

1. What is the primary function of the CRISPR-Cas9 system in gene editing?

A. To amplify specific DNA sequences.
B. To cut DNA at specific locations.
C. To deliver genes into cells.
D. To sequence entire genomes.

Correct answer: B

Rationale: The CRISPR-Cas9 system is a powerful tool used in gene editing to precisely target and cut DNA at specific locations within the genome. This system consists of a guide RNA that directs the Cas9 enzyme to the desired DNA sequence, where it creates a double-strand break. This break can then be repaired by the cell's natural DNA repair mechanisms, allowing for the introduction of specific genetic modifications. Options A, C, and D are incorrect as they do not accurately describe the primary function of the CRISPR-Cas9 system in gene editing. Amplifying DNA sequences, delivering genes into cells, and sequencing entire genomes are not the main functions of the CRISPR-Cas9 system, which is primarily focused on precise DNA cutting for targeted genetic modifications.

2. Which of the following is the cytoplasmic organelle that contains fluid-filled tubules for carrying and transporting substances?

A. Endoplasmic reticulum
B. Golgi apparatus
C. Mitochondria
D. Nucleus

Correct answer: A

Rationale: The endoplasmic reticulum is the correct answer. It is the cytoplasmic organelle that contains fluid-filled tubules responsible for carrying and transporting substances within the cell. The endoplasmic reticulum plays a crucial role in protein synthesis, lipid metabolism, and detoxification processes. The Golgi apparatus, although important in modifying, sorting, and packaging proteins for secretion or for use within the cell, does not primarily contain fluid-filled tubules for carrying and transporting substances. Mitochondria are responsible for generating ATP through cellular respiration, not for carrying and transporting substances. The nucleus contains the cell's genetic material and is involved in regulating cellular activities but does not contain fluid-filled tubules for transportation.

3. What effect does doubling the net force applied to an object have on its acceleration, assuming mass remains constant?

A. Acceleration doubles
B. Acceleration is halved
C. Acceleration remains the same
D. Acceleration quadruples

Correct answer: A

Rationale: According to Newton's second law of motion, acceleration is directly proportional to the net force applied to an object when mass is constant. Therefore, if the net force is doubled, the acceleration of the object will also double. This relationship is expressed by the formula F=ma, where F is the net force, m is the mass, and a is the acceleration. When mass is constant, doubling the force applied will result in a proportional doubling of acceleration. Choices B, C, and D are incorrect because doubling the net force does not halve, maintain, or quadruple the acceleration; it directly and proportionally increases the acceleration.

4. Describe the mechanism by which genes are transmitted from parents to offspring.

A. Blending of parental genes, resulting in an average of their traits.
B. Random assortment of alleles during meiosis, leading to unique combinations in each offspring.
C. Inheritance of solely dominant alleles, masking the influence of recessive ones.
D. Direct transfer of both parental genomes, creating identical copies of the parents.

Correct answer: B

Rationale: A) Blending of parental genes, resulting in an average of their traits, is not an accurate description of how genes are transmitted. In reality, genes are not blended but rather passed down in discrete units. B) Random assortment of alleles during meiosis is the correct mechanism by which genes are transmitted from parents to offspring. During meiosis, homologous chromosomes separate, and alleles are randomly distributed to the gametes, leading to unique combinations of genes in each offspring. C) Inheritance of solely dominant alleles, masking the influence of recessive ones, is not an accurate representation of gene transmission. Offspring inherit alleles from both parents, and the expression of dominant or recessive traits depends on the specific combination of alleles. D) Direct transfer of both parental genomes, creating identical copies of the parents, is not how genes are transmitted. Offspring inherit a unique combination

5. A rock has a mass of 3 grams (g) and a volume of 4 cm³. What is its density?

A. 8.90 g/cm³
B. 0.38 g/cm³
C. 77.22 g/cm³
D. 2.65 g/cm³

Correct answer: D

Rationale: Density is determined by the formula Density = Mass / Volume. For this rock, the mass is 3g, and the volume is 4 cm³. Applying the formula: Density = 3g / 4cm³ = 0.75 g/cm³. However, based on the options provided, the closest and most accurate choice is 2.65 g/cm³, corresponding to option D. Choice A, 8.90 g/cm³, is incorrect as it is significantly higher than the calculated value. Choice B, 0.38 g/cm³, and Choice C, 77.22 g/cm³, are also incorrect and do not match the correct calculation.