Nursing Elites

1. What is the primary function of the CRISPR-Cas9 system in gene editing?

• A. To amplify specific DNA sequences.
• B. To cut DNA at specific locations.
• C. To deliver genes into cells.
• D. To sequence entire genomes.

Correct answer: B

Rationale: The CRISPR-Cas9 system is a powerful tool used in gene editing to precisely target and cut DNA at specific locations within the genome. This system consists of a guide RNA that directs the Cas9 enzyme to the desired DNA sequence, where it creates a double-strand break. This break can then be repaired by the cell's natural DNA repair mechanisms, allowing for the introduction of specific genetic modifications. Options A, C, and D are incorrect as they do not accurately describe the primary function of the CRISPR-Cas9 system in gene editing. Amplifying DNA sequences, delivering genes into cells, and sequencing entire genomes are not the main functions of the CRISPR-Cas9 system, which is primarily focused on precise DNA cutting for targeted genetic modifications.

2. In the K-capture process, a type of electron capture, from which electron shell does the electron get captured?

• A. The outermost s-orbital
• B. An inner p-orbital
• C. An inner d-orbital
• D. Any available electron shell

Correct answer: B

Rationale: The K-capture process involves the capture of an electron from the innermost electron shell, known as the K-shell. The K-shell comprises s and p orbitals. During the K-capture process, an electron is specifically captured from an inner p-orbital within the K-shell. Choices A, C, and D are incorrect because K-capture involves capturing an electron from the innermost shell (K-shell) which consists of s and p orbitals, not the outermost s-orbital, inner d-orbital, or any available electron shell.

3. Which of the following enzymes unwinds the double-stranded DNA during replication?

• A. Helicase
• B. Ligase
• C. Nuclease
• D. Polymerase

Correct answer: A

Rationale: Helicase is the enzyme responsible for unwinding the double-stranded DNA during replication by breaking the hydrogen bonds between the base pairs. This process creates the replication fork where DNA polymerase can then synthesize new DNA strands. Ligase functions to join Okazaki fragments on the lagging strand, not unwind DNA. Nuclease is involved in DNA repair by removing damaged DNA segments. Polymerase is responsible for synthesizing new DNA strands based on the existing template strands, not for unwinding the DNA.

4. Which part of the male reproductive system is responsible for producing seminal fluid?

• A. Bladder
• B. Urethra
• C. Prostate gland
• D. Kidney

Correct answer: C

Rationale: The correct answer is the prostate gland (Choice C). The prostate gland, located near the bladder, is responsible for producing seminal fluid. This fluid, along with sperm from the testes, makes up semen. The bladder stores urine, the urethra is a tube that carries urine from the bladder out of the body, and the kidney filters waste from the blood to produce urine. The prostate gland's specific function is to secrete a milky, alkaline fluid that mixes with sperm during ejaculation to form semen. This fluid helps nourish and protect the sperm, aiding in their motility and viability. Therefore, the prostate gland plays a crucial role in male reproductive function by contributing to the composition and quality of semen, essential for fertility.

5. What is the difference between polygenic inheritance and pleiotropy?

• A. Polygenic inheritance involves multiple genes influencing one trait, while pleiotropy involves one gene affecting multiple traits.
• B. Polygenic inheritance is found in simple Mendelian traits, while pleiotropy is found in complex traits.
• C. Polygenic inheritance is always quantitative, while pleiotropy can be qualitative or quantitative.
• D. Both involve multiple genes and multiple traits, but the specific mechanisms differ

Correct answer: A

Rationale: - Polygenic inheritance refers to the situation where a trait is influenced by multiple genes, each contributing a small effect to the phenotype. These traits often show continuous variation and are not easily categorized into discrete categories. - Pleiotropy, on the other hand, occurs when a single gene influences multiple, seemingly unrelated traits. This means that a mutation in one gene can lead to multiple phenotypic effects across different traits or characteristics. - Therefore, the key difference between polygenic inheritance and pleiotropy lies in the number of genes involved in influencing a trait (multiple genes in polygenic inheritance vs. one gene in pleiotropy) and the direction of influence (one trait affected by multiple genes in polygenic inheritance vs. multiple traits affected by one gene in pleiotropy).

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