HESI RN
Biology Practice Test
1. Which of the following do prokaryotic cells lack?
- A. A cell membrane
- B. Genetic material
- C. Cytoplasm
- D. Membrane-bound organelles
Correct answer: D
Rationale: The correct answer is D: Prokaryotic cells lack membrane-bound organelles, such as mitochondria or a nucleus, distinguishing them from eukaryotic cells. Prokaryotic cells do have genetic material, a cell membrane, and cytoplasm. Choice A is incorrect because prokaryotic cells do have a cell membrane, which is essential for maintaining cell structure and regulating what enters and exits the cell. Choice B is incorrect because prokaryotic cells contain genetic material, typically in the form of a single circular chromosome. Choice C is incorrect because prokaryotic cells have cytoplasm, where various cellular activities take place.
2. What is the composition of the cellular membrane?
- A. A bilayer of phospholipid molecules
- B. A single layer of phospholipid molecules
- C. Proteins that act as transport highways
- D. Carbohydrates
Correct answer: A
Rationale: The cellular membrane is primarily composed of a phospholipid bilayer, which consists of two layers of phospholipid molecules. These molecules have a hydrophilic (water-attracting) head and hydrophobic (water-repelling) tail, creating a barrier that separates the internal cellular environment from the external surroundings. While proteins are embedded within this bilayer and act as transport channels, receptors, and structural support, carbohydrates are found on the outer surface of the membrane for cell recognition and signaling purposes. Therefore, the correct answer is a bilayer of phospholipid molecules.
3. Which of the following molecules contains the code required for replication?
- A. Deoxyribonucleic acid (DNA)
- B. Transfer RNA (tRNA)
- C. Messenger RNA (mRNA)
- D. Ribonucleic acid (RNA)
Correct answer: A
Rationale: The correct answer is A: Deoxyribonucleic acid (DNA). DNA contains the genetic information essential for replication, ensuring the accurate transmission of genetic material from one generation to the next. Choice B, Transfer RNA (tRNA), is involved in protein synthesis, not replication. Choice C, Messenger RNA (mRNA), carries genetic information from DNA to the ribosome but does not contain the code for replication. Choice D, Ribonucleic acid (RNA), is a broader category that includes both mRNA and tRNA, but the specific molecule containing the code required for replication is DNA.
4. In DNA, the nucleotide base adenine always binds with which of the following?
- A. Guanine
- B. Uracil
- C. Thymine
- D. Cytosine
Correct answer: C
Rationale: The correct answer is C: Thymine. In DNA, adenine always pairs with thymine, forming a base pair held together by hydrogen bonds. Guanine pairs with cytosine. Uracil is found in RNA, not DNA. Cytosine pairs with guanine. Therefore, option C is the correct pairing for adenine in DNA.
5. What is the main objective of the translation stage of protein synthesis?
- A. To produce amino acids
- B. To produce nucleotides
- C. To produce fatty acids
- D. To produce proteins
Correct answer: D
Rationale: The main objective of the translation stage of protein synthesis is to produce proteins. During translation, ribosomes decode mRNA sequences to synthesize proteins by linking amino acids together. Choice A, 'To produce amino acids,' is incorrect as amino acids are the building blocks of proteins and are not produced during translation. Choices B and C, 'To produce nucleotides' and 'To produce fatty acids,' are also incorrect as these molecules are not the direct products of the translation stage of protein synthesis.
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